This European Standard specifies a method for the determination of patulin in apple juices and apple puree using high performance liquid chromatography (HPLC). The method has been validated for the determination of patulin via the analysis of naturally contaminated and spiked samples in clear apple juice at levels ranging
from 26 µg/l up to 128 µg/l, in cloudy apple juice at levels ranging from 26 µg/l up to 106 µg/l and in apple puree at levels ranging from 23 µg/kg up to 121 µg/kg.