This European Standard specifies a method for the determination of fumonisin B1 (FB1) and fumonisin B2 (FB2) in maize using high performance liquid chromatography (HPLC). The method has been successfully validated in an interlaboratory study according to AOAC Guidelines for Collaborative Studies [1] on maize containing 405 µg/kg to 6732 µg/kg fumonisin B1 and 152 µg/kg to 2619 µg/kg fumonisin B2. The method works well with maize or minimally processed maize (e.g. fresh, dried and milled maize), but does not provide reliable results with most maize-based processed products.